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Significant growth inhibition by angiotensin 1-7 required daily dosing of the cultures. If the overall calculated charge is zero, the peptide can be diluted with organic solvents (methanol, ethanol, isopropanol or acetonitrile).

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Two deuterium-labeled internal standards (triply deuterated GHRP-4 and GHRP-2 metabolite) were used to optimize the extraction and analysis procedure.

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